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sterilized by autoclaving
immunodiffusion
See 'The X-gal caution in neural transplantation studies' on PubMed
Streptomycin is a protein synthesis inhibitor that binds to the small 16S rRNA of the 30S subunit of the bacterial ribosome
Murashige and Skoog Medium with Vitamins Buffered Hormones sterilized by autoclavingMurashige and Skoog Medium is buffered with MES. MES is an excellent buffer for plant media because of high buffer capacity. Sucrose, Cytokines, Vitamins, and Auxins should be added to the Murashige and Skoog Medium as needed. Buffered Murashige and Skoog Medium is provided as 1 unit to make 10L, 25L, or 50L of medium.
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