FireGene Universal Plant Nuclei Isolation Kit for Single-Nucleus Sequencing DNA Extraction What impact does repeated freezing

What impact does repeated freezing and thawing have

Can the preservation time be extended to 72 hours if the operation cannot be carried out on time due to special circumstances

when resuspending the cell pellet with RPMI 1640 containing 2% FBS

cell counter Storage -80 °C Shelf-life 12 months Kit Components

FireGene Universal Plant Nuclei Isolation Kit for Single-Nucleus Sequencing DNA Extraction What impact does repeated freezingFireGene Universal Plant Nuclei Isolation Kit is designed for the isolation and extraction of cell nuclei from cryopreserved tissue samples of higher plants. The kit uses surfactants to release nuclei, remove chloroplasts, and maintain nuclear membrane stability. After filtration, centrifugation, density layer separation, and chloroplast removal, the workflow generates clean single nucleus suspensions suitable for single nucleus transcriptome