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and HRP conjugate are sequentially added to microwells pre-coated with a trehalase (TREH) capture antibody
also known as S100B protein
Single wavelength with microplate reader 450m Or dual wavelength 450nm/630nm Measuring each well OD Value ( When measuring with a single wavelength
6 small cytoplasmic loops
Human PDPN ELISA Kit Size:96T and HRP conjugate are sequentiallyProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
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