Acidic Protease Microplate Assay Kit Other Cytokines and the absorbance value was

and the absorbance value was measured by microplate reader at 450 nm detection wavelength (correction wavelength 570-630 nm)

After trypan blue staining

One unit will cleave ≥95% of 1µg of recombinant monoclonal IgG in 30 minutes at 37°C

(2014) CSF-1R Signaling in Health and Disease: A Focus on the Mammary Gland

Acidic Protease Microplate Assay Kit Other Cytokines and the absorbance value wasProduct Specification Detection Type Colorimetric Method General Notes The Dye in the kit is easy to oxidise and deteriorate after dissolving, so it needs to be mixed and used on the spot, and is not recommended for storage. Instructions Urine and serum, plasma and tissue extract, cell lysis liquid, cell culture supernatant, other biological liquid samples. Storage Temp. Transportation and storage at 4 , 6months Test Range 0. 05 mmol L 5 mmol L