Termination: Add 50uL of stop solution to each well to terminate the reaction (blue immediately turns yellow at this time)
(3) Centrifuge for 5 minutes to discard the supernatant
application dilution species WB 1:500 IHC-P 1:500 IF 1:500
Light sensitivity:This reagent is sensitive to light and will accelerate the attenuation of luminous intensity if exposed to light during storage
Biotinylated EGFRvIII His&Avi Tag Protein, Human Size:100μg Termination: Add 50uL of stopProduct Specification Species Human Synonyms EGFRvIII, ERBB, ERBB1, HER1, mENA, NISBD2, PIG61 Accession NP_001333870. 1 Amino Acid Sequence Leu25 Ser378, with C terminal His and Avitag Expression System HEK293 Molecular Weight 60 75kDa (Reducing) Purity 95% by SDS PAGE Endotoxin <1EU g Conjugation Biotin Tag Avi Tag, His Tag Physical Appearance Lyophilized Powder Storage Buffer PBS, pH7. 4. Reconstitution Reconstitute at 0. 1 1 mg ml according to the