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Three samples of known concentration were tested on 1 ELISA plate for 20 The assay was repeated 40 times to assess intra-plate precision
The intensity of the color is positively correlated with the amount of alpha-klotho protein (α-klotho) in the sample
acetylation
three extracellular immunoglobulin domains of the C2-type for antibody binding
Human FCRL3 ELISA Kit Size:96T Three samples of known concentrationProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
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